Number of RNAPol I per rRNA gene in CHO cell

Value 114 polymerases/gene
Organism Mammalian tissue culture cell
Reference French SL, Osheim YN, Cioci F, Nomura M, Beyer AL. In exponentially growing Saccharomyces cerevisiae cells, rRNA synthesis is determined by the summed RNA polymerase I loading rate rather than by the number of active genes. Mol Cell Biol. 2003 Mar23(5):1558-68. p.1567 left column 2nd paragraphPubMed ID12588976
Primary Source Puvion-Dutilleul F, Bachellerie JP. Ribosomal transcriptional complexes in subnuclear fractions of Chinese hamster ovary cells after short-term actinomycin D treatment. J Ultrastruct Res. 1979 Feb66(2):190-9.PubMed ID430587
Comments "...average Pol I density has been measured on rRNA genes from CHO cells (primary source) and rat liver cells (15) and found to be 114 polymerases/gene and 101 polymerases/gene, respectively, which correspond to one polymerase/123 nt and one polymerase/139 nt."
Entered by Uri M
ID 108306