Volumetric measurement of cells and cell components

Range Table - link μm^3
Organism Budding yeast Saccharomyces cerevisiae
Reference Yamaguchi M, Namiki Y, Okada H, Mori Y, Furukawa H, Wang J, Ohkusu M, Kawamoto S. Structome of Saccharomyces cerevisiae determined by freeze-substitution and serial ultrathin-sectioning electron microscopy. J Electron Microsc (Tokyo). 2011 60(5):321-35. doi: 10.1093/jmicro/dfr052 p.326 table 2PubMed ID21908548
Method P.322 left column 2nd paragraph: "Here, [Researchers] report the structome of S. cerevisiae strain S288c determined by freeze substitution and serial ultrathin-sectioning electron microscopy."
Comments P.327 right column bottom paragraph: "The nucleus, nuclear envelope, nuclear pore and nucleolus: The nucleus was enclosed by a double-layered nuclear envelope (Fig. 1i). The nucleus in interphase cell was spherical (Fig. 1i), ∼1.6 µm in diameter (Table 3) and ∼1.8 µm^3 in volume, occupying ∼10.5% of the cell volume (Table 2, Fig. 4). The nuclear envelope consisted of the outer and inner envelopes. Both measured ∼13 nm in thickness (Table 4). The nuclear pore was ∼94 nm in diameter (Table 3) and is known to function in the transport of substances between the nucleoplasm and cytoplasm [ref 15]. There was only one nucleolus in a nucleus (Table 1). The nucleolus was composed of densely packed granular materials (Fig. 1i) ∼0.4 µm^3 in volume and occupied ∼22% of the nuclear volume (Table 2)." P.329 right column bottom paragraph: "The volume of cytosol was calculated by subtracting the volume of all organelles and cell components (from cell wall to multivesicular bodies, but not nucleolus, in Table 2) from the cell volume. Cytosol occupied ~64% of the cell volume (Table 2, Fig. 4)." P.330 right column 6th paragraph: "The nucleus, nuclear envelope, nuclear pore and nucleolus: The nucleus was ~1.3 μm^3 in volume and occupied ~10% of the cell volume (Table 2, Fig. 3), whereas the nucleolus was ~0.2 μm^3 in volume and occupied ~16% of the nuclear volume (Table 2)." See notes beneath table
Entered by Uri M
ID 108294