||Budding yeast Saccharomyces cerevisiae
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||P.1727 right column bottom paragraph: "For the SAGE method, the error associated with a value increases with a decreasing number of transcripts per cell. The conclusions drawn from this study are dependent on the quality of the mRNA levels from previously published data (ref 35). Since more than 65% of the mRNA levels included in this study were calculated to 10 copies/cell or less (40% were less than 4 copies/cell), the error associated with these values may be quite large. The mRNA levels were calculated from more than 20,000 transcripts. Assuming that the estimate of 15,000 mRNA molecules per cell is correct (primary source), this would mean that mRNA transcripts present at only a single copy per cell would be detected 72% of the time (ref 35). The mRNA levels for each gene were carefully scrutinized, and only mRNA levels for which a high degree of confidence existed were included in the correlation value."