Steady-state intracellular concentrations of measured metabolites from aerobic glucose-limited cultures at D=0.1/hour

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Canelas AB, van Gulik WM, Heijnen JJ. Determination of the cytosolic free NAD/NADH ratio in Saccharomyces cerevisiae under steady-state and highly dynamic conditions. Biotechnol Bioeng. 2008 Jul 1 100(4):734-43. p.738 table 2PubMed ID18383140
Method The concentrations of the metabolic intermediates G6P, F6P, FBP, PEP, pyruvate, T6P, 6PG, G1P, M6P, oxoglutarate, succinate, fumarate, malate, and M1P, as well as the combined pools 2PG+3PG and citrate+isocitrate, were determined by ESI-LC-MS/MS (Van Dam et al., 2002). Quantification was based on IDMS (Mashego et al., 2004 Wu et al., 2005). The concentrations of NAD and NADH were determined by the spectrophotometric cycling method as described in Visser et al. (2004).
Comments As shown in Table II, no significant differences were observed in the levels of all 17 measured metabolites, including NAD and NADH, between the two strains.
Entered by Uri M
ID 108141