| Comments |
P.288 right column bottom paragraph: "The presence of these compounds in
cultures of S. cerevisiae is probably
not a result of cell lysis. This can be
concluded from the fact that in cultures of both S. cerevisiae and C.
utilis only 15-20 mg/l of protein was
found in the culture fluid under steady-state conditions (at a cell
concentration of 6.25 g/l). Assuming
that this protein is due to lysis of 40
mg of cells and that the specific cell
volume is 2 ml/g cells, the intracellular concentration of, for
example, 2-oxoglutarate should have been as high as 750 mM." Specific cell volume, which is the ratio of cell volume per cell dry weight, is not constant but depends on growth rate and physiology. |