| Value |
1.4
μm/min
Range: ±0.4 μm/min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol. 2006 Sep8(9):957-62. p.960 left column top paragraphPubMed ID16906145
|
| Primary Source |
Tanaka K, Mukae N, Dewar H, van Breugel M, James EK, Prescott AR, Antony C, Tanaka TU. Molecular mechanisms of kinetochore capture by spindle microtubules. Nature. 2005 Apr 21 434(7036):987-94.PubMed ID15846338
|
| Method |
To determine whether budding yeast Kip3p is a microtubule
depolymerase, the full-length His-tagged protein was expressed in
insect cells, purified to homogeneity and its activity measured in
sedimentation assays. |
| Comments |
Because their speed was faster
than the microtubule growth rate of 1.4±0.4µm/min (primary source), Kip3p
spots always caught up with the ends of growing microtubules, leading
to bright plus-end labelling (Fig. 4). See p.960 right column top paragraph for "typical microtubule growth
rates of ~1µm/min" |
| Entered by |
Uri M |
| ID |
108075 |