Microtubule growth rate

Value 1.4 μm/min Range: ±0.4 μm/min
Organism Budding yeast Saccharomyces cerevisiae
Reference Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol. 2006 Sep8(9):957-62. p.960 left column top paragraphPubMed ID16906145
Primary Source Tanaka K, Mukae N, Dewar H, van Breugel M, James EK, Prescott AR, Antony C, Tanaka TU. Molecular mechanisms of kinetochore capture by spindle microtubules. Nature. 2005 Apr 21 434(7036):987-94.PubMed ID15846338
Method To determine whether budding yeast Kip3p is a microtubule depolymerase, the full-length His-tagged protein was expressed in insect cells, purified to homogeneity and its activity measured in sedimentation assays.
Comments Because their speed was faster than the microtubule growth rate of 1.4±0.4µm/min (primary source), Kip3p spots always caught up with the ends of growing microtubules, leading to bright plus-end labelling (Fig. 4). See p.960 right column top paragraph for "typical microtubule growth rates of ~1µm/min"
Entered by Uri M
ID 108075