Kinesin-like protein KIF2C depolymerizes the minus end faster than the plus end by

Value 1.7 Fold Range: ±0.2 Fold
Organism Budding yeast Saccharomyces cerevisiae
Reference Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol. 2006 Sep8(9):957-62. p.958 left column bottom paragraphPubMed ID16906145
Method To determine whether budding yeast Kip3p is a microtubule depolymerase, the full-length His-tagged protein was expressed in insect cells, purified to homogeneity and its activity measured in sedimentation assays.
Comments Although MCAK acts at both ends, it depolymerizes the minus end 1.7±0.2 times faster than the plus end (mean ± s.d., n = 26).
Entered by Uri M
ID 108074