Effect of the carbon source on the concentrations of metabolites

Range Table - link
Organism Bacteria Escherichia coli
Reference Bautista J, Satrústegui J, Machado A. Evidence suggesting that the NADPH/NADP ratio modulates the splitting of the isocitrate flux between the glyoxylic and tricarboxylic acid cycles, in Escherichia coli. FEBS Lett. 1979 Sep 15 105(2):333-6. p.335 table 4PubMed ID39785
Method "Samples (100 or 200 ml) of E. coli cultures were rapidly gathered in pre-cooled recipients and immediately immersed in liquid nitrogen."
Comments "[Investigators] have measured the cellular concentrations of several metabolites whose variations in glucose and acetate-grown cells could give some insight into the actual occurrence of NADPH inhibition of E. coli IDH [NADP-isocitrate dehydrogenase]. The results are shown in table 4. The 5-fold increase in citrate and the 2-fold decrease in oxoglutarate found in acetate-grown cells is consistent with an actual inhibition of IDH. According to [investigators'] expectations, the NADPH/NADP ratio rises from 6.2-14 in glucose and acetate-grown cells, respectively, thus supporting the regulatory role of this parameter for the operation of the glyoxylate cycle."
Entered by Uri M
ID 108042