Diffusion coefficient of CheY-GFP

Value 4.6 μm^2/sec Range: ±0.8 Table - link μm^2/sec
Organism Bacteria Escherichia coli
Reference Kumar M, Mommer MS, Sourjik V. Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli. Biophys J. 2010 Feb 17 98(4):552-9. p.557 right column 3rd paragraphPubMed ID20159151
Primary Source Cluzel P, Surette M, Leibler S An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells. Science. 2000 Mar 3 287(5458):1652-5.PubMed ID10698740
Method Researchers used fluorescence recovery after photobleaching (FRAP) along with a detailed numerical model that simulates FRAP experiments, to systematically investigate mobility of cytoplasmic, inner membrane, and DNA-binding proteins in Escherichia coli.
Comments The diffusion coefficient of CheY-GFP (40 kDa) estimated by fluorescence correlation spectroscopy was 4.6±0.8µm^2/sec (primary source)
Entered by Uri M
ID 107989