| Value |
5.6
Amino acids/second
|
| Organism |
Mouse Mus musculus |
| Reference |
Ingolia NT, Lareau LF, Weissman JS. Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes. Cell. 2011 Nov 11 147(4):789-802 doi: 10.1016/j.cell.2011.10.002. p.793 left column bottom paragraphPubMed ID22056041
|
| Method |
(P.790 left column 2nd paragraph:)"(1) Generation of cell extracts in which ribosomes have
been faithfully halted along the mRNA they are translating in vivo
(2) nuclease digestion of RNAs that are not protected by the
ribosome followed by recovery of the ribosome-protected
mRNA fragments (3) quantitative conversion of the protected
RNA fragments into a DNA library that can be analyzed by
deep sequencing (Ingolia, 2010: Ingolia et al., 2009: Lau et al., 2001: Pfeffer et al., 2005)." |
| Comments |
"Metagene analyses revealed a progressive depletion of ribosomes from the 5’ to the 3’ ends of the messages after harringtonine [a drug] treatment. Following a delay of ~60 s, which presumably reflects the time required for engagement of harringtonine, ribosomes
progress from the 5' ends of transcripts at a rate of 5.6 amino
acids per second (Figures 3B and 3C), which is consistent
with values from previous single-gene measurements (Bostro¨m
et al., 1986 [PMID 3020051])." |
| Entered by |
Uri M |
| ID |
107952 |