Mutation rate

Range 0.8e-8 mutations/site/generation
Organism Nematode Caenorhabditis elegans
Reference Baer CF, Miyamoto MM, Denver DR. Mutation rate variation in multicellular eukaryotes: causes and consequences. Nat Rev Genet. 2007 Aug8(8):619-31. p.623 right column top paragraphPubMed ID17637734
Method "Mutation-accumulation (MA) lines: Laboratory experiments on evolution in which populations are forced into a bottleneck for many generations to allow for the very nearly neutral accumulation of mutations over time offer a powerful approach to studying mutation processes. A survey of mutations in a long-term set of C. elegans MA lines revealed a mutation rate (2.1×10^-8 per site per generation) that is approximately one order of magnitude greater than the average of previous reporter gene estimates for this species [Denver et al., PMID 15295601]. This approach relied on PCR and direct DNA sequencing to survey numerous genomic regions for mutation in the nematode MA lines, which [investigators] believe provides a more accurate and direct measure of the mutation rate. A similar approach, this time applying a denaturing high-performance liquid chromatography (DHPLC) mutation pre-screen to three genetically distinct sets of Drosophila MA lines [Haag-Liautard C, et al. PMID 17203060], bolstered the initial findings in C. elegans by showing a similarly high mutation rate: 0.8×10^-8 per site per generation."
Entered by Uri M
ID 107886