Characteristics of an oscillating culture of S. cerevisiae in continuous cultivation at D=0.22hour^-1
Range | Table - link |
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Organism | Budding yeast Saccharomyces cerevisiae |
Reference | Hans MA, Heinzle E, Wittmann C. Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae. Biotechnol Bioeng. 2003 Apr 20 82(2):143-51. p.148 table 1PubMed ID12584756 |
Method | Amino acids were derivatized with ortho-phthaldialdehyde (OPA), separated by HPLC using a Grom-Sil OPA-3 column (125 × 4 mm, Grom-Analytik, Herrenberg, Germany) and detected as isoindol-derivatives by fluorescence (Agilent Series 1100 Agilent Technologies, Waldbronn, Germany) with excitation at 330 nm and emission at 450 nm. |
Entered by | Uri M |
ID | 107765 |