Protein content

Range ~15 mg protein/gram FW
Organism Thale cress Arabidopsis thaliana
Reference Piques et al., Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis. Mol Syst Biol. 2009 5: 314 p.4 left column top paragraphPubMed ID19888209
Primary Source Gibon Y et al., Adjustment of diurnal starch turnover to short days: depletion of sugar during the night leads to a temporary inhibition of carbohydrate utilization, accumulation of sugars and post-translational activation of ADP-glucose pyrophosphorylase in the following light period. Plant J. 2004 Sep39(6):847-62. AND Gibon Y et al., Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environ. 2009 Jul32(7):859-74 AND Hannemann J, Poorter H, Usadel B, Bla¨sing OE, Finck A, Tardieu F, Atkin OK, Pons T, Stitt M, Gibon Y (2009) Xeml Lab: a tool that supports the design of experiments at a graphical interface and generates computer-readable meta-data files, which capture information about genotypes, growth conditions, environmental perturbations and sampling strategy. Plant Cell Environ 32: 1185–1200PubMed ID15341628, 19236606, 19236607
Method Two complementary approaches were taken to quantify cytosolic and plastid ribosomes: The first approach used qRT–PCR. The second approach used relative quantitative proteomics based on emPAI values (Ishihama et al, 2005) to estimate the abundance of ribosomal proteins in the polysome gradient fractions.
Entered by Uri M
ID 107724