||Miura F, Kawaguchi N, Yoshida M, Uematsu C, Kito K, Sakaki Y, Ito T. Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs. BMC Genomics. 2008 Nov 29 9: 574. p.10 left column top paragraphPubMed ID19040753
||P.10 left column top paragraph: "The median lengths of 5'/3'-untranslated regions revealed by tiling array hybridization [ref 26] and RNA-Seq [ref 27] were reported to be 68 nt/91 nt and 50 nt/104 nt, respectively. Thus, the average length of mRNA is 1,500 nt, coincident with the previous estimate [ref 19]. However, it should be noted that a significant negative correlation was observed between expression level and ORF length in the budding yeast [ref 28]. Indeed, the average size of the yeast ORFs can be as short as 1,123 nt and 1,083 nt when weighted by the copy numbers calculated from signal intensity of high-density DNA microarray [ref 29] and GATC-PCR results, respectively. Thus, the average length of mRNA should be regarded as ~1,250 nt rather than 1,500 nt."