Number of ribosomes in HeLa cell

Range ~3,300,000 Ribosomes/cell
Organism Human Homo sapiens
Reference Duncan R, Hershey JW. Identification and quantitation of levels of protein synthesis initiation factors in crude HeLa cell lysates by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem. 1983 Jun 10 258(11):7228-35. p.7233 left column bottom paragraphPubMed ID6853516
Method Ribosome levels were measured by quantitative precipitation of cytoplasmic ribosomes and ribosomal subunits, phenol extraction, and determination of the mass of 28 S rRNA by sucrose gradient centrifugation and absorbance measurements.
Entered by Uri M
ID 107552