pH in cytosol of Human endothelial cell ECV304

Value 7.59 pH units Range: ±0.01 pH units
Organism Human Homo sapiens
Reference Porcelli AM, Ghelli A, Zanna C, Pinton P, Rizzuto R, Rugolo M. pH difference across the outer mitochondrial membrane measured with a green fluorescent protein mutant. Biochem Biophys Res Commun. 2005 Jan 28 326(4):799-804. p.801 right column 2nd paragraph and p.802 fig.3PubMed ID15607740
Method Researchers generated a EYFP targeted to mitochondrial intermembrane space IMS (MIMS-EYFP) to determine for the first time the pH within this mitochondrial compartment in intact cells. A similar calibration was carried out also in cells transfected with cyt-EYFP and with mt- EYFP.
Comments n=21. The cytosol pH value obtained with EYFP is very similar to that determined from experiments with the pH-sensitive dye BCECF (7.51±0.15, n=5).
Entered by Uri M
ID 107273