| Value |
7.78
pH units
Range: ±0.17 pH units
|
| Organism |
Human Homo sapiens |
| Reference |
Porcelli AM, Ghelli A, Zanna C, Pinton P, Rizzuto R, Rugolo M. pH difference across the outer mitochondrial membrane measured with a green fluorescent protein mutant. Biochem Biophys Res Commun. 2005 Jan 28 326(4):799-804. doi:10.1016/j.bbrc.2004.11.105 p.801 right column 2nd paragraphPubMed ID15607740
|
| Method |
"[Researchers] generated a EYFP targeted to mitochondrial intermembrane space IMS (MIMS-EYFP) to determine for the first time the pH within this mitochondrial compartment in intact cells." |
| Comments |
"When the average values of fluorescence in the selected areas were plotted against pH, the calibration line shown in Fig. 2B was obtained. A similar calibration was carried out also in cells transfected with cyt-EYFP and with mt-EYFP (data not shown). With this calibration, the pH values obtained were 6.88 ± 0.09 (n = 32) in the IMS, 7.78 ± 0.17 (n = 9) in the matrix, and 7.59 ± 0.01 (n = 21) in the cytosol, as illustrated in Fig. 3." |
| Entered by |
Uri M |
| ID |
107272 |