Time for DsRed to reach half maximal red fluorescence in HeLa cell

Range ~27 Hours
Organism Unspecified
Reference Baird GS, Zacharias DA, Tsien RY. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 2000 Oct 24 97(22):11984-9. p.11986 left column top paragraphPubMed ID11050229
Method "Quantum yields for photodestruction were separately measured on a microscope stage or in a spectrofluorimeter." "For time trials of DsRed maturation, a dilute solution of freshly synthesized and purified DsRed was made in 10mMTris, pH 8.5, and this solution was stored at room temperature in a stoppered cuvette (not airtight) and subjected to periodic spectral analysis. For mutant maturation data, fluorescence emission spectra (excitation at 475 nm or 558 nm) were taken directly after synthesis and purification, and then after .2 months storage at 4°C or room temperature."
Comments Red fluorescence reaches half its maximal fluorescence after approximately 27 h and requires >48 h to reach >90% of maximal fluorescence.
Entered by Uri M
ID 107006