Maturation time of enhanced green fluorescent protein (EGFP)

Value 94 Min Range: Table - link Min
Organism Bacteria Escherichia coli
Reference Iizuka R, Yamagishi-Shirasaki M, Funatsu T. Kinetic study of de novo chromophore maturation of fluorescent proteins. Anal Biochem. 2011 Jul 15 414(2):173-8. p.176 table 1PubMed ID21459075
Primary Source Evdokimov et al., Structural basis for the fast maturation of Arthropoda green fluorescent protein. EMBO Rep. 2006 Oct7(10):1006-12.PubMed ID16936637
Method Reoxidation rate of GFP with a reduced chromophore at 25°C.
Comments Note-5650sec (94 min) given by Reference. The primary source, p.1010 table 1 gives maturation half time of 3,915sec (65min). Discrepancy unclear.
Entered by Uri M
ID 107002