Diffusion coefficients of S1-Qdot on membranes of live bacteria

Range 0.46 to 0.04 before lysis: 7.29 after cell lysis μm^2/sec
Organism Bacteria Escherichia coli
Reference Leptihn S, Har JY, Chen J, Ho B, Wohland T, Ding JL. Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria. BMC Biol. 2009 May 11 7: 22. p.6 of 13 left column bottom paragraphPubMed ID19432949
Method Using fluorescence correlation spectroscopy (FCS), researchers measured in real-time the diffusion of 100 nM S1-Qdot conjugates upon binding to the immobilized bacteria, which have been attached to a glass slide by 0.01% poly-L-lysine.
Comments At the end of 1 h, the addition of SDS lysed the bacteria, releasing the AMPQdot into the supernatant (Figure 4A). These particles have D = 7.29 µm^2/sec which is comparable to that of free Qdot diffusion in solution, and approximately 18 times faster than that of bound particles (fig.4B)
Entered by Uri M
ID 106971