Maturation time of mKO fluorescent protein

Value 4.5 Hours Range: Table - link Hours
Organism Bacteria Escherichia coli
Reference Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 Dec2(12):905-9. Article link - link Supplementary table 1 PubMed ID16299475
Primary Source Karasawa et al., 2004, see ref 10 beneath table
Method (From Karasawa et. al:) "[Researchers] utilized ... two FPs as a novel donor/acceptor pair for FRET (fluorescence resonance energy transfer) measurements detecting caspase 3 activity during an apoptotic process. The performance of this donor/acceptor pair is highly effective, possibly proving to be as useful an experimental system as the common CFP/YFP (yellow FP) pair."
Entered by Uri M
ID 106882