Median RNAP II density inside the genes

Value 0.078 Molecules/kb
Organism Budding yeast Saccharomyces cerevisiae
Reference Pelechano V, Chávez S, Pérez-Ortín JE. A complete set of nascent transcription rates for yeast genes. PLoS One. 2010 Nov 16 5(11):e15442. p.5 left column 2nd paragraphPubMed ID21103382
Method Researchers developed genomic variants of the well-known run-on technique to evaluate the nascent TR (transcription rate) for most genes. In this technique (GRO, Genomic Run-on), elongating RNA pol molecules, that conserve the RNA, are forced to incorporate radioactive UTP for a short length.
Comments By assuming the known datum that RNA polymerase molecules transcribe at 25 nt/s [Edwards et al., 1991 PMID 1985924], then the median RNA pol II density inside the genes is 0.078 molecules/kb. Corresponds to an average of 0.096 molecules/gene.
Entered by Uri M
ID 106768