CRP-RNAP interaction energy

Range -3.26±0.41 kcal/mol
Organism Bacteria Escherichia coli
Reference Kinney JB, Murugan A, Callan CG Jr, Cox EC. Using deep sequencing to characterize the biophysical mechanism of a transcriptional regulatory sequence. Proc Natl Acad Sci U S A. 2010 May 18 107(20):9158-63 p.9161 right column 4th paragraph and fig.3BPubMed ID20439748
Method Using I(tµ) evaluated on full-wt data, researchers fit all of the parameters defining t, including ei, Cc, and the elements of the energy matrices used to compute ec and er .
Comments Researchers inferred a CRP-RNAP interaction energy ei=-3.26±0.41kcal/mol. This value is consistent with Kuhlman et al.'s (2007 PMID 17376875) measurement of -3.4kcal/mol (for TK310 cells).
Entered by Uri M
ID 106623