Halflife of aspartate transcarbamoylase (PyrB) in exponentially growing cells with ammonium as the nitrogen source

Value 1.5 Hours
Organism Bacteria Bacillus subtilis
Reference Gerth U, Kock H, Kusters I, Michalik S, Switzer RL, Hecker M. Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol. 2008 Jan190(1):321-31 p.329 right column top paragraphPubMed ID17981983
Primary Source Bond RW, Field AS, Switzer RL. Nutritional regulation of degradation of aspartate transcarbamylase and of bulk protein in exponentially growing Bacillus subtilis cells.J Bacteriol. 1983 Jan153(1):253-8.PubMed ID6401278
Method (Primary source abstract:) Measurement of enzyme activity after the addition of uridine to repress further enzyme synthesis and specific immunoprecipitation of the enzyme from cells grown in the presence of [3H]leucine
Comments Supplementation with certain amino acids blocked this rapid turnover.
Entered by Uri M
ID 106565