Density of cells arrested in various stages of the cell cycle

Range 1.0846 G1: 1.1049 S: 1.0998 metaphase g/mL
Organism Budding yeast Saccharomyces cerevisiae
Reference Bryan AK, Goranov A, Amon A, Manalis SR. Measurement of mass, density, and volume during the cell cycle of yeast. Proc Natl Acad Sci U S A. 2010 Jan 19 107(3):999-1004 p.1000 right column bottom paragraphPubMed ID20080562
Method "To determine how cell density correlates with the cell cycle, [researchers] measured the distributions of buoyant mass and volume in budding yeast populations." Cells arrested in G1 by treatment with the pheromone alpha factor...Cells arrested in S phase by the replication inhibitor hydroxyurea...cells arrested in metaphase by the microtubule inhibitor nocodazole (NOC)"
Comments Cells arrested in G1 1.0846±0.0043g/mL. Cells arrested in S phase 1.1049±0.0024g/mL. Cells arrested in metaphase 1.0998±0.0049g/mL. The density of asynchronous budding yeast in researchers' strain background, W303, was 1.1029±0.0026g/mL. See BNID 103876
Entered by Uri M
ID 106439