Cellular transcription rate of Mdm2 predicted by model

Range >500 mRNAs/hour
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column bottom paragraphPubMed ID21593866
Method P.337 right column top paragraph: "Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives [ref 10]." See model in p.339 fig. 3a
Comments P.339 left column bottom paragraph: "Average cellular transcription rates predicted by the model spanned two orders of magnitude with a median of about two mRNA molecules per hour [Fig. 3b]. An extreme example was Mdm2 with more than 500 mRNAs per hour." NIH3T3 (mouse fibroblast) cells.
Entered by Uri M
ID 106380