Number of ubiquitin and H2A histones (each) in HeLa cell

Value 1.5e+8 Molecules/cell
Organism Human Homo sapiens
Reference Carlson N, Rechsteiner M. Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions. J Cell Biol. 1987 Mar104(3):537-46. p.544 left column 2nd paragraphPubMed ID3029141
Method Injection of radioiodinated ubiquitin to HeLa cells
Comments Based on a DNA content of 17 pg per cell (Lin et al., 1964 PMID 14262469), researchers estimate that a HeLa cell contains 1.5×10^8 copies of H2A, hence an equivalent number of ubiquitin molecules.This is equivalent to a concentration of 150µM (according to BNID 104 519) however Pielak et al., PMID 19113834 p.229 right column 6th paragraph cite ref above and give value of ~10µM. See Clague et al. 2015 PMID 25906909 p.2 right column top paragraph:"...Ub-histone H2A (UbH2A) ... is one of the most abundant cellular proteins (~1.7×10^7 copies per cell, HeLa)."
Entered by Uri M
ID 106197