Estradiol levels

Range Non breeder 9.3 to 28.9: breeder 14.4 to 71.1 pg/ml
Organism Mouse Mus musculus
Reference Oury F et al., Endocrine Regulation of Male Fertility by the Skeleton. Cell. 2011 Mar 4 144(5):796-809 p.797 right column 4th paragraphPubMed ID21333348
Method P.807 left column bottom paragraph: "Sperm Counts and Hormone Measurements: Caudal epididymides were minced in 1 ml PBS, and the number of cells released was counted after 1 hr. The total sperm count was assessed in the final suspension by using a hemocytometer (Dakhova et al., 2009). Circulating levels of testosterone, estradiol (E2), and progesterone were measured by radioimmunoassay (RIA) from Diagnostic Systems Laboratories (Testosterone RIA DSL-4000, Estradiol RIA DSL-43100, and Progesterone RIA-3900)."
Comments P.797 right column 4th paragraph: "Circulating progesterone levels were similar in Ocn−/− and WT mice, and although circulating levels of estradiol were higher in Ocn−/− than in WT mice, they remained within the normal range (from 9.3 to 28.9 ng/ml for nonbreeder mice and from 14.4 to 71.1 ng/ml for breeder mice) (Figure 2K). Estradiol levels were not affected in Esp−/− mice. As predicted by the coculture assays, female fertility, ovary weight, morphology of the uterus, follicles number, and circulating levels of sex steroid hormones were normal in Ocn−/− female mice (Figures S1D–S1L)." In the original paper (BioNumbers was notified) there was a typo and thus reported as ng/ml instead of pg/ml.
Entered by Uri M
ID 106170