Concentration of vimentin in KD (human fibroblast) cell

Value 151 µg/ml Range: Estimated 10,000µm filament/cell µg/ml
Organism Mammalian tissue culture cell
Reference Luby-Phelps K. Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area. Int Rev Cytol. 2000 192: 189-221. p.195 top paragraphPubMed ID10553280
Primary Source Lai YK, Lee WC, Chen KD. Vimentin serves as a phosphate sink during the apparent activation of protein kinases by okadaic acid in mammalian cells. J Cell Biochem. 1993 Oct53(2):161-8. p.163 table 1 link - link PubMed ID7693732
Method (primary source:) immunoblotting and scanning densitometry
Comments Concentration in 'Value' from primary source. Estimated length in 'Range' from reference: "...This concentration results in an estimated 10,000 µm filament per cell (based on 32 monomers per 10 nm diameter ×48 nm long association domain)." Cells from rat and human tissue.
Entered by Uri M
ID 105909