Fraction of protein in carboxysome that is RubisCO

Value 62 %
Organism Bacteria Thiobacillus neapolitanus
Reference G. C. Cannon and J. M. Shively Characterization of a homogenous preparation of carboxysomes from Thiobacillus neapolitanus, Arch. Microbiol.1983. 134(1): 52–59 p. 56 right column and table 2 (bands 5-8 and 12 identified as large and small subunits of RubisCO)
Method Dissociation of purified carboxysomes with SDS in the presence of ß-mercaptoethanol and subsequent electrophoresis on SDS polyacrylamide gels polymerized from 15%w/v acrylamide
Comments As much as 80 % of the ribulose-1,5-bisphosphate carboxylase- oxygenase (RubisCO 3-phospho-D-glycerate carboxylase [dimerizing], EC activity of Thiobacillus neapolitanus can be found within polyhedral inclusion bodies called carboxysomes (Shively et al., 1973 PMID 4355679 Beudeker et al., 1980 Arch Microbiol 124: 185-189). See BNID 101680
Entered by Uri M
ID 105882