Spread surface area of CCL39 hamster fibroblast

Value 1900 µm^2
Organism Mammalian tissue culture cell
Reference Boulter E, Grall D, Cagnol S, Van Obberghen-Schilling E. Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase. FASEB J. 2006 Jul20(9):1489-91 p.E643 fig 1DPubMed ID16723384
Method P.1490 caption to figure 1D: "The mean surface area (±sem, n>60) was determined on F-actin-stained cells 20 h after plating on fibronectin-coated coverslips."
Comments P.E642 right column 5th paragraph: "The most striking phenotype of ILK-GFP-F-[a chimeric molecule comprising ILK [Integrin-linked kinase] fused to a membrane-targeted green fluorescent protein] expressing fibroblasts was their increased size. The surface area of spread ILK-GFP-F cells plated on fibronectin was 1.82 fold larger than that of ILK-GFP cells (Fig. 1D), suggesting that membrane targeting alone is able to activate the cell machinery involved in cell spreading." Value extracted visually from figure
Entered by Uri M
ID 105877