Charge/mass (m/z) ratio of immonium ion of phosphotyrosine

Value 216.043 unitless
Organism Generic
Reference Mann M, Ong SE, Grønborg M, Steen H, Jensen ON, Pandey A. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 2002 Jun20(6):261-8. p.265 left column top sentencePubMed ID12007495
Primary Source Steen H, Küster B, Mann M. Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning. J Mass Spectrom. 2001 Jul36(7):782-90. AND Steen H, Küster B, Fernandez M, Pandey A, Mann M. Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal Chem. 2001 Apr 1 73(7):1440-8.PubMed ID11473401, 11321292
Method A precursor-ion scanning method that can be performed in the positive mode has recently been developed for the specific detection of phosphotyrosine-containing peptides [primary sources]. This method is based on the ability to selectively detect the immonium ions of phosphotyrosine residues that have an m/z value of 216.043. Immonium ions are generated by double cleavage of the peptide backbone.
Entered by Uri M
ID 105738