Diffusion coefficient of EGFP in nucleoplasm

Value 27.9 µm^2/sec Range: ±2.2 µm^2/sec
Organism Human Homo sapiens
Reference Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.78 table 1 Table - link PubMed ID20921136
Method FCS (fluorescence correlation spectroscopy)-a technique that focuses a laser beam on a spot of interest in the nucleoplasm and monitors fluctuations in fluorescence intensity over time. The intensity record is transformed into an autocorrelation function that assesses the diffusion correlation time of the detected molecules.
Entered by Uri M
ID 105696