Diffusion coefficient of EGFP in nucleoplasm
| Value | 27.9 µm^2/sec Range: ±2.2 µm^2/sec |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.78 table 1 Table - link PubMed ID20921136 |
| Method | FCS (fluorescence correlation spectroscopy)-a technique that focuses a laser beam on a spot of interest in the nucleoplasm and monitors fluctuations in fluorescence intensity over time. The intensity record is transformed into an autocorrelation function that assesses the diffusion correlation time of the detected molecules. |
| Entered by | Uri M |
| ID | 105696 |