Intracellular free and total calcium as a function of extracellular [Ca2+] in control and energy-depleted cells

Range Table - link
Organism Bacteria Escherichia coli
Reference Gangola P, Rosen BP. Maintenance of intracellular calcium in Escherichia coli. J Biol Chem. 1987 Sep 15 262(26):12570-4. p. 12572 table 1PubMed ID2442165
Method Free intracellular calcium was determined from fura-2 fluorescence and using the following equation: [Ca2+]i= Kd(F - Fmin)/(Fmax - F) where F is the fluorescence intensity of dye in the cells, Fmax and Fmin are the intensities at saturating and zero calcium concentrations, respectively, and Kd is the dissociation constant for fura-2 determined under the appropriate conditions.
Comments The cytosolic concentration of free Ca2+ in the unstarved E . coli cells was determined to be 90±9nM (n = 6). See column 2 in table for intracellular [Ca2+]. See BNID 100130.
Entered by Uri M
ID 105669