||Sea urchin Strongylocentrotus purpuratus
||Howard-Ashby M, Materna SC, Brown CT, Chen L, Cameron RA, Davidson EH. Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development. Dev Biol. 2006 Dec 1 300(1):74-89 p. 82 table 2 Table - link PubMed ID17055477
||QPCR was used to determine the expression profile of each identified
transcription factor from unfertilized egg to 48 h. mRNA was isolated from egg,
6, 12, 18, 24, 36 and 48 h embryos with the Sigma GenElute Mammalian Total
RNA Miniprep Kit, per the manufacturers instructions.
||As expected, there was
much variation among the half-lives of different transcripts.
Sp-nk7 had the shortest half-life, at 3.30 h, while Sp-pax2/5/8 had the longest, at 31.4 h. The average half-life of any
message was 12.9 h, about twice the 5.7-h average half-life
for total polysomal RNA transcript in the S. purpuratus
blastula–gastrula embryo (Davidson, 1986). The rate of
synthesis of different mRNAs was likewise quite variable,
ranging from 36 molecules/h (Sp-lhx2) to 550 molecules/h
(Sp-irxA). The average rate of synthesis was about 150
molecules/h per embryo.