Percent of rapidly labeled mRNA associated with polysomes that is not polyadenylated

Range 20-35 %
Organism Eukaryotes
Reference Schröder HC, Friese U, Bachmann M, Zaubitzer T, Müller WE. Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei. Eur J Biochem. 1989 Apr 15 181(1):149-58. p. 149 left columnPubMed ID2565812
Primary Source 1) Katinakis PK, Slater A, Burdon RH. Non-polyadenylated mRNAs from eukaryotes. FEBS Lett. 1980 Jul 11 116(1):1-7 AND (2) Bernd A, Schröder HC, Zahn RK, Müller WE. Modulation of the nuclear-envelope nucleoside triphosphatase by poly(A)-rich mRNA and by microtubule protein. Eur J Biochem. 1982 Dec129(1):43-9.PubMed ID6997068, 6130941
Comments A major portion of the RNA transported out of nuclei, is known to lack 3'-end poly(A) sequences [poly(A)-free mRNA] and hence to be unable to stimulate the NTPase. About 20-35% of the rapidly labeled mRNA associated with polysomes has been shown to be nonpolyadenylated or to contain only small oligo(A) sequences, not binding to oligo(dT)-celIulose [primary source 1], incapable of stimulating the NTPase [primary source 2].
Entered by Uri M
ID 105619