| Range |
10000-20000 proteins/cell
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Clarke DC, Betterton MD, Liu X. Systems theory of Smad signalling. Syst Biol (Stevenage). 2006 Nov153(6):412-24. p. 415 table 2PubMed ID17186703
|
| Method |
Researchers estimated the number of cellular Smad proteins by
quantitative immunoblotting of Mv1Lu cell lysates. Quantitative
immunoblots were performed by comparing the band intensities
of cell lysates to a standard curve of recombinant
protein dilutions. |
| Comments |
Researchers observed that Smad2 and
Smad4 (BNID 105616) were present in similar amounts, whereas Smad3
was present in amounts 4–5 times less than Smads 2 (BNID 105614) and
4. This finding was consistent across several cell lines. |
| Entered by |
Uri M |
| ID |
105615 |