Concentration of Smad2 transcription factor in Mv1Lu (mink lung epithelial cells)

Range 85000-120000 proteins/cell
Organism Mammalian tissue culture cell
Reference Clarke DC, Betterton MD, Liu X. Systems theory of Smad signalling. Syst Biol (Stevenage). 2006 Nov153(6):412-24. p. 415 table 2PubMed ID17186703
Method Researchers estimated the number of cellular Smad proteins by quantitative immunoblotting of Mv1Lu cell lysates. Quantitative immunoblots were performed by comparing the band intensities of cell lysates to a standard curve of recombinant protein dilutions.
Comments Researchers observed that Smad2 and Smad4 (BNID 105616) were present in similar amounts, whereas Smad3 (BNID 105615) was present in amounts 4–5 times less than Smads 2 and 4. This finding was consistent across several cell lines.
Entered by Uri M
ID 105614