| Comments |
P.1123 left column top paragraph: "In the past 30 years, a wide array of biochemical, molecular and imaging approaches have been used to estimate the elongation rate of RNA polymerase II (Pol II) in vivo (Table 1). Although these studies have provided rates that were critical in evaluating the mechanics and regulation of gene expression in different model organisms, their focus has primarily been on single genes and, in some cases, on non-native, engineered transcription cassettes. In an article on page 1128 of this issue (ref 1), Singh and Padgett examine the elongation rate of Pol II as well as the splicing kinetics of pre-mRNA at up to ten large, native genes in human cells. Interestingly, they show that the average elongation rate at the tested genes is 3.8 kb/min [BNID 105566], considerably higher than most previous estimates (Table 1). The attractive method described in this paper could potentially be applied generally to determine the rate of transcription and splicing kinetics at most Pol II–transcribed genes in different eukaryotic model systems." Table gives values from different studies in range of 1.1-6kb/min equivalent to 18-100b/sec. See BNID 101904, 100662, 105565 |