Average golgi vesicle life time in ovary gland cell

Value ~8.86 min
Organism Plant Aptenia cordifolia
Reference Kristen U, Lockhausen J. Estimation of Golgi membrane flow rates in ovary glands of aptenia cordifolia using cytochalasin B. Eur J Cell Biol. 1983 Jan29(2):262-7.PubMed ID6832170
Method Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the plasma membrane. The ultrastructural feature of the Golgi apparatus in control and cyt B treated cells was analyzed using stereological methods in order to estimate the mean area of vesicular membrane produced by a single dictyosome during a 30 min period of effective cyt B action.
Comments The turnover time of a single Golgi cisterna was determinated to be 7.34 min, and the average vesicle life time to be 8.86 min.
Entered by Uri M
ID 105563