| Range |
6.93-9.9 hours
|
| Organism |
Cyanobacteria Synechocystis PCC 6803 |
| Reference |
Shastri AA, Morgan JA. Flux balance analysis of photoautotrophic metabolism. Biotechnol Prog. 2005 Nov-Dec21(6):1617-26. p. 1619 left column 3rd paragraphPubMed ID16321043
|
| Method |
Autotrophic Growth Conditions. A 1-L culture of
Synechocystis was cultivated autotrophically in a 1.25-L
bioreactor (Bioflo 3000, New Brunswick Scientific, NJ)
under a surface light flux of 150 µE/m^2/s in BG-11
medium (American Type Culture Collection medium 616)
buffered with 10 mM HEPES under atmospheric levels
of carbon dioxide at 30°C. The pH was controlled at 7.5±0.1. Growth was monitored spectrophotometrically by
measuring the optical absorbance of cultures at 600 and
730 nm. |
| Comments |
To perform simulations of
growth, it is necessary to add constraints for maximum
nutrient and light uptake rates. The maximum growth
rate of photoautotrophically cultivated Synechocystis was
measured to be 0.085±0.015 h^-1 from four separate
experiments. This value is consistent with other reported
values (Emlyn-Jones et al., 1999 PMID 10476038). Generation time calculated from G=ln2/k where G is the generation time and k is the growth rate. See BNID 100289 for generation time of 6 hours. |
| Entered by |
Uri M |
| ID |
105522 |