||Archaea Nanoarchaeum equitans
||Waters E et al., The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc Natl Acad Sci U S A. 2003 Oct 28 100(22):12984-8. abstract & p. 12985 left column bottom paragraphPubMed ID14566062
||"Genomic DNA was either digested with restriction enzymes
or sheared to provide clonable fragments. Two plasmid
libraries were made by subcloning randomly sheared fragments
of this DNA into a high-copy number vector (˜2.8 kbp library)
or low-copy number vector (˜6.3 kbp library). DNA sequence
was obtained from both ends of plasmid inserts to create
‘‘mate-pairs,’’ pairs of reads from single clones that should be
adjacent to one another in the genome. Library construction,
DNA sequencing, and assembly methods were essentially as
described (refs 9–11 in article)."
||"The N. equitans genome (490,885 base pairs) encodes the machinery for information processing and repair, but lacks genes for lipid, cofactor, amino acid, or nucleotide biosyntheses." p. 12984 right column top paragraph: "The assembly procedures resulted in a single
scaffold of four contigs comprising 489,082 base pairs. The gaps
between the four contigs were then sequenced, resulting in a
single circular sequence." p. 12985 left column bottom paragraph: "The genome of N. equitans (GenBank accession no.
AACL01000000) consists of a single, circular chromosome of 490,885 bp and has an average G+C content of 31.6%."