Turnover number of 3 sodium-hydrogen exchanger (NHE) isoforms calculated from Vmax, measured by fluorometry at 37°C, and amount of NHE at cell surface of PS120 fibroblasts

Range Table - link
Organism Chinese hamster
Reference Cavet ME, Akhter S, de Medina FS, Donowitz M, Tse CM. Na(+)/H(+) exchangers (NHE1-3) have similar turnover numbers but different percentages on the cell surface. Am J Physiol. 1999 Dec277(6 Pt 1):C1111-21. p. C1119 Table 2PubMed ID10600762
Method A Chinese hamster lung fibroblast cell line (PS120) previously selected to lack all endogenous NHEs was stably transfected, using Lipofectin (GIBCO BRL), with human NHE1 or rabbit NHE2 or NHE3 cDNAs epitope tagged on the COOH termini with a previously described 11-amino acid epitope of vesicular stomatitis virus glycoprotein (VSVG) plus an 8-amino acid spacer (NHE1V, NHE2V, and NHE3V). Researchers then determined the turnover numbers of NHE1, NHE2, and NHE3 expressed in the same cell type (PS120) by correlating the Vmax (as measured by 22Na+ uptake kinetic studies and fluorometry) with the amount of NHE protein expressed at the cell surface.
Entered by Uri M
ID 105480