Method |
See notes under table for calculations of radius and volume fluctuations. ? (right column) obtained by using the rms(dv) values and Eq. 4 at 25°C: rms(dV)=(kT/2?)×ro. Suppose a protein molecule is considered as a sphere that is isotropically
compressible up to a certain minimum size ro, ? is the proportionality constant
and formally represents the interfacial tension between
the protein and the solvent, and k and T are the Boltzmann constant and the absolute
temperature, respectively. V is volume. |