Rate of CO2 evolution in E. coli grown on acetate

Value 150 mmol/min/liter cell water Range: ±7 Table - link mmol/min/liter cell water
Organism Bacteria Escherichia coli
Reference Walsh K, Koshland DE Jr. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. J Biol Chem. 1984 Aug 10 259(15):9646-54. p.9648 table IIPubMed ID6378912
Method Measurements made after isotopic steady state was reached.
Comments 150mM/min measured directly. 174mM/min calculated when the following velocities are summed (See fig. 1 in article): V(CO2)=V(KGDH, a-ketoglutarate dehydrogenase)+V(IDH, isocitrate dehydrogenase)+VPEPCK(phosphoenolpyruvate carboxykinase)+V(malic enzyme). 23559 (Met-) strain
Entered by Uri M
ID 105257