Km of isocitrate lyase for isocitrate

Range 0.6-3 mM
Organism Bacteria Escherichia coli
Reference E.M.T. El-Man, H.G. Nimmo and W-H. Helms, The role of isocitrate in control of the phosphorylation of isocitrate dehydrogenase in Escherichia coli ML308, FEBS 2456 April 1985 Volume 183, number 2
Primary Source 1) Bautista J, Satrústegui J, Machado A. Evidence suggesting that the NADPH/NADP ratio modulates the splitting of the isocitrate flux between the glyoxylic and tricarboxylic acid cycles, in Escherichia coli. FEBS Lett. 1979 Sep 15 105(issue 2):333-6 (2) Walsh K, Koshland DE Jr. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. J Biol Chem. 1984 Aug 10 259(15):9646-54.PubMed ID39785, 6378912
Comments 3mM from primary source (1). 0.6mM from primary source (2). Note: Watanabe et al PMID 15470117, Table - link give value of 0.65 mM at 40°C (Optimum temperature for activity) and 0.25mM at 10°C. See BNID 105320
Entered by Uri M
ID 105252