Glutathione (GSSG) level in Chang's liver cell incubated with a cytokine mixture

Value 4.75 nmol/mg protein
Organism Mammalian tissue culture cell
Reference Crespo I et al., Differential effects of dietary flavonoids on reactive oxygen and nitrogen species generation and changes in antioxidant enzyme expression induced by proinflammatory cytokines in Chang Liver cells. Food Chem Toxicol. 2008 May46(5):1555-69. doi: 10.1016/j.fct.2007.12.014. p.1557 figure 2APubMed ID18234413
Method P.1557 right column bottom paragraph to p.1558 left column: "Two spectrophotometry cuvettes per sample were prepared with 1.8 mL phosphate-EDTA buffer, 100 µL supernatant and 100 µL O-phthalaldehyde (7.5 µM). After incubating for 15 min at 4 °C, a spectrofluorometric reading was obtained at an excitation wavelength of 350 nm and an emission wavelength of 420 nm." P.1558 left column: "GSH was obtained by subtracting GSSG from total glutathione." Value extracted visually from fig. 2A
Comments For GSSG level without cytokine mixture see BNID 102531.
Entered by Uri M
ID 105115