Ribosomal GTP hydrolysis, dipeptide formation and translocation times

Range Table - link
Organism Bacteria Escherichia coli
Reference Bilgin N, Claesens F, Pahverk H, Ehrenberg M. Kinetic properties of Escherichia coli ribosomes with altered forms of S12. J Mol Biol. 1992 Apr 20 224(4):1011-27. p.1020 table 2PubMed ID1569565
Method P.1012 left column 3rd paragraph: "A quench-flow device with a time resolution of about one millisecond is used to start elongation by rapidly mixing initiated ribosomes with ternary complex and to quench the reaction at different time points after mixing. With this technique researchers have characterized different steps during the translation cycle of wild-type ribosomes, such as GTP hydrolysis on EF-Tu and dipeptide formation. [Researchers] have used the same technique to study the time evolution of the first few elongation cycles of poly(Phe) synthesis. [They] have, in particular, investigated how mutations in the ribosomal protein S12 or the presence of Sm influence different steps in the ribosomal elongation cycle."
Comments An E. coli translation rate of 19.2 aa/sec is given in table. See BNID 100059, 100233, 105067
Entered by Uri M
ID 105068